In Vitro Assay
Checkerboard Synergy Analysis
How two compounds interact across a matrix of concentrations — synergy, additivity, indifference, or antagonism.
A checkerboard analysis evaluates how two antimicrobial agents interact when combined, mapping their effect across a matrix of concentrations. It identifies whether a combination is synergistic, additive, indifferent, or antagonistic.
For programs built around combination therapy, this is essential evidence. TransPharm performs checkerboard assays against our validated pathogen library, including multidrug-resistant strains, to characterize combinations that may extend or restore antimicrobial activity.
Methodology
- Two-agent concentration matrix
- Fractional inhibitory concentration (FIC) index
- Testing against target and MDR strains
- Synergy / additivity / antagonism classification
Applications
- Evaluating combination therapy candidates
- Identifying synergy against resistant organisms
- Screening adjuvant and potentiator compounds
- Supporting combination dosing rationale
Customized to your program
Every assay is tailored to your compound and your panel of organisms, including the resistant phenotypes most relevant to your program. Contact us to learn more about in vitro efficacy testing of your test article, or to request a free, no-obligation quote.