In Vitro Assay
Minimum Inhibitory Concentration (MIC) Testing
The lowest concentration of your compound that inhibits visible growth of a pathogen — the cornerstone susceptibility measurement.
A minimum inhibitory concentration (MIC) assay determines the lowest concentration of drug needed to inhibit the growth of a pathogen. It is the foundational measurement of antimicrobial potency and the starting point for most compound development programs.
TransPharm offers MIC assays using both agar and broth dilution methods according to CLSI (Clinical & Laboratory Standards Institute) guidelines. Our on-site library contains hundreds of validated pathogens, including many multidrug-resistant (MDR) strains, and additional strains can be obtained upon request.
Methodology
- Broth microdilution (CLSI-standard)
- Broth macrodilution
- Agar dilution
- Testing against single isolates or large panels
Applications
- Comparative potency ranking of lead candidates
- Baseline susceptibility against target and resistant strains
- Screening compound libraries against defined panels
- Establishing activity ahead of in vivo efficacy studies
Customized to your program
Every assay is tailored to your compound and your panel of organisms, including the resistant phenotypes most relevant to your program. Contact us to learn more about in vitro efficacy testing of your test article, or to request a free, no-obligation quote.