In Vitro Assay
Minimum Bactericidal Concentration (MBC) Testing
The lowest concentration that kills a pathogen, not just inhibits it — distinguishing bactericidal activity from bacteriostatic.
A minimum bactericidal concentration (MBC) assay determines the lowest concentration of a compound required to kill a pathogen, rather than simply inhibit its growth. Where the MIC measures inhibition, the MBC measures true kill.
TransPharm determines MBC by subculturing from MIC assay wells that show no visible growth, quantifying the concentration that achieves a defined reduction in viable organisms. This distinguishes bactericidal compounds from bacteriostatic ones — a distinction that matters for many therapeutic indications.
Methodology
- Subculture from MIC wells
- Viable count determination
- CLSI-aligned methodology
- Paired with MIC for a complete potency profile
Applications
- Distinguishing bactericidal from bacteriostatic activity
- Characterizing compounds for indications requiring kill
- Supporting candidate selection decisions
- Complementing MIC data in a full susceptibility package
Customized to your program
Every assay is tailored to your compound and your panel of organisms, including the resistant phenotypes most relevant to your program. Contact us to learn more about in vitro efficacy testing of your test article, or to request a free, no-obligation quote.